The Stagecoach co-founder, her husband and two members of her family strongly deny the allegations.
Scottish transport tycoon Dame Ann Gloag has been charged with human trafficking offences.
Her husband David McCleary and two other members of their family have also been charged.
BBC Scotland has been told that the 80-year-old co-founder of Stagecoach was charged after voluntarily attending Livingston police station in West Lothian for an interview.
All four strongly deny the charges against them.
A statement issued on behalf of Dame Ann said: “Whilst we cannot comment on the details of an ongoing investigation, Dame Ann Gloag strongly disputes the malicious allegations that have been made against her, her foundation and members of her family, and will vigorously defend herself and the work of her foundation to protect her legacy and continue her work helping thousands of people in the UK and abroad every year.”
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